1. SEKU Repository
  2. Journal Articles
  3. School of Science and Computing (JA)
Please use this identifier to cite or link to this item: https://repository.seku.ac.ke/handle/123456789/3515
Title: Ten (10) M Ammonium acetate is an efficient molecular concentration for the extraction of genomic DNA from small insects used for rolling circle amplification
Authors: Muturi, S. M.
Karanja, L. S.
Wachira, Francis N.
Wambulwa, Moses C.
Keywords: Pseudococcidae
Aphididae
Auchenorryncha
DNA extraction
rolling circle amplification
restriction analysis
Issue Date: Sep-2013
Publisher: Academic Journals
Citation: African Journal of Biotechnology, Vol. 12(39), pp. 5790-5798, 25 September, 2013
Abstract: Most of the published methods for DNA isolation from vectors (insects) are not always effective in mealy-bugs, aphids and leafhoppers because they have high amount of polyphenols in their body systems. A simple, rapid, reliable labor-effective and carcinogenic compound-free salting out method for isolation of high molecular DNA from these insects is described. The ratio of OD260/OD280 ranged between 1.8 to 2.0. The method was successfully applied to Rolling Circle Amplification and in restriction analysis, indicating the removal of the common inhibitors.
Description: DOI: 10.5897/AJB2013.12848
URI: https://www.researchgate.net/publication/263850157_Ten_10_M_Ammonium_acetate_is_an_efficient_molecular_concentration_for_the_extraction_of_genomic_DNA_from_small_insects_used_for_rolling_circle_amplification
http://repository.seku.ac.ke/handle/123456789/3515
ISSN: 1684-5315
Appears in Collections:School of Science and Computing (JA)

Files in This Item:
File Description SizeFormat 
Muturi_Ten (10) M Ammonium acetate is....pdffulltext616.09 kBAdobe PDFThumbnail
View/Open
Show full item record


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.