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Please use this identifier to cite or link to this item: https://repository.seku.ac.ke/handle/123456789/3515
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dc.contributor.authorMuturi, S. M.
dc.contributor.authorKaranja, L. S.
dc.contributor.authorWachira, Francis N.
dc.contributor.authorWambulwa, Moses C.
dc.date.accessioned2017-08-23T09:00:50Z
dc.date.available2017-08-23T09:00:50Z
dc.date.issued2013-09
dc.identifier.citationAfrican Journal of Biotechnology, Vol. 12(39), pp. 5790-5798, 25 September, 2013en_US
dc.identifier.issn1684-5315
dc.identifier.urihttps://www.researchgate.net/publication/263850157_Ten_10_M_Ammonium_acetate_is_an_efficient_molecular_concentration_for_the_extraction_of_genomic_DNA_from_small_insects_used_for_rolling_circle_amplification
dc.identifier.urihttp://repository.seku.ac.ke/handle/123456789/3515
dc.descriptionDOI: 10.5897/AJB2013.12848en_US
dc.description.abstractMost of the published methods for DNA isolation from vectors (insects) are not always effective in mealy-bugs, aphids and leafhoppers because they have high amount of polyphenols in their body systems. A simple, rapid, reliable labor-effective and carcinogenic compound-free salting out method for isolation of high molecular DNA from these insects is described. The ratio of OD260/OD280 ranged between 1.8 to 2.0. The method was successfully applied to Rolling Circle Amplification and in restriction analysis, indicating the removal of the common inhibitors.en_US
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.subjectPseudococcidaeen_US
dc.subjectAphididaeen_US
dc.subjectAuchenorrynchaen_US
dc.subjectDNA extractionen_US
dc.subjectrolling circle amplificationen_US
dc.subjectrestriction analysisen_US
dc.titleTen (10) M Ammonium acetate is an efficient molecular concentration for the extraction of genomic DNA from small insects used for rolling circle amplificationen_US
dc.typeArticleen_US
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