Genetic diversity and population structure of wild and cultivated crotalaria species based on genotyping-by-sequencing

Show simple item record Muli, Joshua K. Neondo, Johnstone O. Kamau, Peter K. Michuki, George N. Odari, Eddy Budambula, Nancy L. 2022-09-06T12:47:34Z 2022-09-06T12:47:34Z 2022-09-01
dc.identifier.citation PLoS ONE September1, 20222 en_US
dc.identifier.issn 1932-6203
dc.description en_US
dc.description.abstract Crotalaria is a plant genus that is found all over the world, with over 700 species of herbs and shrubs. The species are potential alternative food and industrial crops due to their adaptability to different environments. Currently, information on the genetic diversity and population structure of these species is scanty. Genotyping-by-sequencing (GBS) is a cost-effective high-throughput technique in diversity evaluation of plant species that have not been fully sequenced. In the current study, de novo GBS was used to characterize 80 Crotalaria accessions from five geographical regions in Kenya. A total of 9820 single nucleotide polymorphism (SNP) markers were obtained after thinning and filtering, which were then used for the analysis of genetic diversity and population structure in Crotalaria. The proportion of SNPs with a minor allele frequency (maf) > = 0.05 was 45.08%, while the Guanine-Cytosine (GC) content was 0.45, from an average sequence depth of 455,909 reads per base. The transition vs transversion ratio was 1.81 and Heterozygosity (He) ranged between 0.01–0.07 in all the sites and 0.04 to 0.52 in the segregating sites. The mean Tajima’s D value for the population was -0.094, suggesting an excess of rare alleles. The fixation index (Fst) between the different populations based on the Wright Fst (1943) ranged from 0.0119 to 0.066 for the Eastern-Western and Nairobi-Western populations. Model based techniques of population structure analysis including structure, k-means and cross-entropy depicted eight clusters in the study accessions. Non-model based techniques especially DAPC depicted poor population stratification. Correspondence Analysis (CA), Principal coordinate analyses (PCoA) and phylogenetic analysis identified a moderate level of population stratification. Results from this study will help conservationists and breeders understand the genetic diversity of Crotalaria. The study also provides valuable information for genetic improvement of domesticated species. en_US
dc.language.iso en en_US
dc.publisher Public Library of Science en_US
dc.subject Population genetics en_US
dc.subject Domestic animals en_US
dc.subject Domestic animals en_US
dc.subject Valleys en_US
dc.subject Species diversity en_US
dc.subject Kenya en_US
dc.subject Heterozygosity en_US
dc.subject Plant breeding en_US
dc.title Genetic diversity and population structure of wild and cultivated crotalaria species based on genotyping-by-sequencing en_US
dc.type Article en_US

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