Rapid and efficient plant regeneration from shoot apical meristems of finger millet [Eleusine coracana (L.) Gaertn.] via direct organogenesis

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dc.contributor.author Mukami, Asunta
dc.contributor.author Ngetich, Alex
dc.contributor.author Mweu, Cecilia
dc.contributor.author Muthangya, Mutemi
dc.contributor.author Oduor, Richard O.
dc.contributor.author Ngugi, Mathew
dc.contributor.author Mbinda, Wilton
dc.date.accessioned 2018-08-10T07:58:18Z
dc.date.available 2018-08-10T07:58:18Z
dc.date.issued 2018-07-18
dc.identifier.citation African journal of biotechnology, Vol. 17(29), pp. 898-905, 18 July, 2018 en_US
dc.identifier.issn 1684-5315
dc.identifier.uri http://www.academicjournals.org/journal/AJB/article-full-text-pdf/3EBE05657934
dc.identifier.uri http://repository.seku.ac.ke/handle/123456789/4260
dc.description DOI: 10.5897/AJB2018.16562 en_US
dc.description.abstract A simple and efficient plant regeneration system via direct organogenesis was established in finger millet using in vitro derived shoot apical meristems. Six varieties; GBK-043128, GBK-043094, GBK043050, GBK-043137, GBK-043122 and GBK-043124 were evaluated. MS medium was used for cotyledonary germination. Maximum number of shoots (84.33%) was observed in variety GBK-043128 while GBK-043094 had the least germination efficiency (62. 67%). Shoot apical meristems from threeday old seedlings were evaluated for their potency of shoot induction on varied 6-benzylaminopurine (BAP) concentrations. Highest shoot induction was observed in medium supplemented with 1.75 mg/l BAP in GBK-043050 (3.00) whereas GBK-043094 (1.28) had the least response in medium supplemented with 1.0 mg/l BAP. To induce rooting, in vitro regenerated plants cultured on MS medium was supplemented with different concentrations of indole-3- acetic acid. The highest response in root induction, with a larger number of roots (10.28), was observed in MS medium supplemented with 4.0 µM IAA. Statistical analysis indicated that plant regeneration response varied greatly among the varieties. In vitro germinated plants were successfully transferred to the greenhouse after hardening, with 300 shoots developing into fertile plants, which were indistinguishable with wild type plants. This plant regeneration system has potential for production of transgenic finger millet crops. en_US
dc.language.iso en en_US
dc.publisher Academic Journals en_US
dc.subject Direct organogenesis en_US
dc.subject finger millet en_US
dc.subject root induction en_US
dc.subject shoot apical meristems en_US
dc.title Rapid and efficient plant regeneration from shoot apical meristems of finger millet [Eleusine coracana (L.) Gaertn.] via direct organogenesis en_US
dc.type Article en_US

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