Genetic diversity of indigenous Bradyrhizobium nodulating promiscuous soybean [Glycine max (L) Merr.] varieties in Kenya: Impact of phosphorus and lime fertilization in two contrasting sites

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dc.contributor.author Wasike, V. W.
dc.contributor.author Lesueur, D.
dc.contributor.author Wachira, Francis N.
dc.contributor.author Mungai, N. W.
dc.contributor.author Mumera, L. M.
dc.contributor.author Sanginga, N.
dc.contributor.author Mburu, H. N.
dc.contributor.author Mugadi, D.
dc.contributor.author Wango, P.
dc.contributor.author Vanlauwe, B.
dc.date.accessioned 2017-08-25T08:42:23Z
dc.date.available 2017-08-25T08:42:23Z
dc.date.issued 2009-09
dc.identifier.citation Plant and Soil, September 2009, Volume 322, Issue 1–2, pp 151–163 en_US
dc.identifier.issn 0032-079X
dc.identifier.uri https://link.springer.com/content/pdf/10.1007%2Fs11104-009-9902-7.pdf
dc.identifier.uri http://repository.seku.ac.ke/handle/123456789/3530
dc.description DOI 10.1007/s11104-009-9902-7 en_US
dc.description.abstract While soybean is an exotic crop introduced in Kenya early last century, promiscuous (TGx) varieties which nodulate with indigenous rhizobia have only recently been introduced. Since farmers in Kenya generally cannot afford or access fertilizer or inoculants, the identification of effective indigenous Bradyrhizobium strains which nodulate promiscuous soybean could be useful in the development of inoculant strains. Genetic diversity and phylogeny of indigenous Bradyrhizobium strains nodulating seven introduced promiscuous soybean varieties grown in two different sites in Kenya was assayed using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) of the 16S-23S rDNA intergenic spacer region and 16S rRNA gene sequencing. PCR-RFLP analysis directly applied on 289 nodules using Msp I distinguished 18 intergenic spacer groups (IGS) I–XVIII. Predominant IGS groups were I, III, II, IV and VI which constituted 43.9%, 24.6%, 8.3% 7.6% and 6.9% respectively of all the analyzed nodules from the two sites while IGS group VII, IX, X, XI, XII, XIV, XVI, XVII, XVIII each constituted 1% or less. The IGS groups were specific to sites and treatments but not varieties. Phylogenetic analysis of the 16S rRNA gene sequences showed that all indigenous strains belong to the genus Bradyrhizobium. Bradyrhizobium elkanii, Bradyrhizobium spp and Bradyrhizobium japonicum related strains were the most predominant and accounted for 37.9%, 34.5%, and 20.7% respectively while B. yuanmigense related accounted for 6.9% of all strains identified in the two combined sites. The diversity identified in Bradyrhizobium populations in the two sites represent a valuable genetic resource that has potential utility for the selection of more competitive and effective strains to improve biological nitrogen fixation and thus increase soybean yields at low cost. en_US
dc.language.iso en en_US
dc.publisher Springer Verlag en_US
dc.subject Agro-ecological zones en_US
dc.subject Genetic diversity en_US
dc.subject Promiscuous soybean en_US
dc.subject Polymerase chain reaction-restriction fragment length polymorphism en_US
dc.title Genetic diversity of indigenous Bradyrhizobium nodulating promiscuous soybean [Glycine max (L) Merr.] varieties in Kenya: Impact of phosphorus and lime fertilization in two contrasting sites en_US
dc.type Article en_US


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