Tea ( Camellia sinensis ) infusions ameliorate cancer in 4TI metastatic breast cancer model

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dc.contributor.author Mbuthia, Karori S.
dc.contributor.author Mireji, Paul O.
dc.contributor.author Ngure, Raphael M.
dc.contributor.author Stomeo, Francesca
dc.contributor.author Kyallo, Martina
dc.contributor.author Muoki, Chalo
dc.contributor.author Wachira, Francis N.
dc.date.accessioned 2017-04-20T07:17:51Z
dc.date.available 2017-04-20T07:17:51Z
dc.date.issued 2017
dc.identifier.citation BMC Complementary and Alternative Medicine (2017) 17:202 en_US
dc.identifier.uri http://download.springer.com/static/pdf/23/art%253A10.1186%252Fs12906-017-1683-6.pdf?originUrl=http%3A%2F%2Fbmccomplementalternmed.biomedcentral.com%2Farticle%2F10.1186%2Fs12906-017-1683-6&token2=exp=1492672234~acl=%2Fstatic%2Fpdf%2F23%2Fart%25253A10.1186%25252Fs12906-017-1683-6.pdf*~hmac=6b6b9258d0ce842ea15bb8cb6430f40c6805e625d7692f104b6780ba4f6e0cad
dc.identifier.uri http://repository.seku.ac.ke/handle/123456789/3435
dc.description DOI 10.1186/s12906-017-1683-6 en_US
dc.description.abstract Background: Tea ( Camellia sinensis ) infusions are widely consumed beverages with numerous health benefits. However, physiological and molecular responses mediating these activities are poorly understood. Method: Three replicates of 4TI cancer cell suspension (2.0 × 10 5 cells/ml) were challenged in vitro with various concentrations of green, black and purple tea infusions to assese s their cytoxicity and associated differentially expressed genes in the cells. Inhibitory activity was tested by using serial dilutions of respective tea infusions in a 96 well ELISA plate. Results: Green tea had the highest inhibition on 4TI cells proliferation at a concentration of IC 50 = 13.12 μ g/ml. Further analysis of the 4TI cancer cell line treated with tea using 454 pyrosequencing generated 425,696 reads with an input mean length of 286.54. Trimmed sequences were import ed on a CLC genomic workbench v7.03 and annotated on a reference mouse genome ( Mus musculus strain C57BL/6 J ). Results revealed a differential expression of apoptosis related genes in the transcriptome. Casp8, Casp9, Casp3, Casp6, Casp8AP2, Aifm1, Aifm2 and Apopt1 genes were significantly upregulated indicating the process of apoptosis was initiated and executed. Conclusion: These findings on caspases offer valuable informat ion on the mechanism of tea as an anticancer agent and will contribute to further research in future novel treatments. en_US
dc.language.iso en en_US
dc.publisher BioMed Central en_US
dc.subject Catechins en_US
dc.subject EGCG en_US
dc.subject Theaflavins en_US
dc.subject Anthocyanins en_US
dc.subject Antioxidant activity en_US
dc.subject Caspases en_US
dc.title Tea ( Camellia sinensis ) infusions ameliorate cancer in 4TI metastatic breast cancer model en_US
dc.type Article en_US

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