School of Science and Computing (JA)

Isolation and functional profiling of seed‐associated bacteria from rattlepods (crotalaria spp.) with biotechnological potential

Thu, 01 Jan 2026 00:00:00 GMT

Isolation and functional profiling of seed‐associated bacteria from rattlepods (crotalaria spp.) with biotechnological potential Odoi, Brenda A.; Nthiwa, Daniel M.; Neondo, Johnstone O .; Muli, Joshua K.; Kamau, Peter K.; Budambula, Nancy L. Microorganisms associated with plant seeds have recently gained increased attention due to their pivotal role in enhancing plant health through the acquisition of nutrients and plant growth promotion. Although rattlepods (Crotalaria spp.) are widely consumed, information regarding the bacterial diversity and functional roles within their seeds remain unexplored. This study isolated, identified, and characterized endophytic and epiphytic culturable bacteria from seeds of Kenyan rattlepods. A total of 118 seed-associated bacterial isolates were cultured from the rattlepods (Crotalaria spp.), and 20 of them were identified using 16S rRNA gene sequencing. The isolates belonged to two distinct phyla: Proteobacteria and Firmicutes. The isolates belonged to four genera: nine Bacillus, nine Proteus, one Lysinibacillus, and one Morganella. BLAST analysis of partial 16S rRNA gene sequences (763-1273bp) showed that 19 of the 20 isolates exhibited high similarity (˃99%) to previously described species, whereas one isolate showed low similarity (< 0.05) higher protease and cellulase. Isolates 4EnA1/2/1 (P. mirabilis) and 9EnA (P. mirabilis) were the highest significant amylase producers, whereas isolates 3EnB3/3 (Proteus sp. [in enterobacteria]) and 10EnA 2/1 (P. mirabilis) were the highest significant lipase producers. Most seed-associated bacteria isolated from Crotalaria spp. were identified as known species; however, some isolates may represent novel taxa and could serve as promising candidates for the production of biotechnologically important extracellular enzymes under mesophilic conditions. https://doi.org/10.1155/ijm/6560468

Evaluation of cabbage aphid preference for Ethiopian mustard (Brassica carinata): implications for trap cropping in kale systems

Wed, 29 Apr 2026 00:00:00 GMT

Evaluation of cabbage aphid preference for Ethiopian mustard (Brassica carinata): implications for trap cropping in kale systems Mutua, Bretor K.; Adan, Isack H.; Karanja, Edward N.; Dubois, Thomas; Mutyambai, Daniel M. Trap cropping has proven to be a promising, widely documented, and sustainable eco-friendly strategy within integrated pest management systems for managing insect pests. Numerous studies have successfully demonstrated its effectiveness across diverse agricultural systems, with new ones, continually being developed. The current study sought to evaluate the potential of Ethiopian mustard (Brassica carinata) as a trap crop for managing cabbage aphids (Brevicoryne brassicae L.) through laboratory choice tests and field experiments. Volatile organic compounds (VOCs) emission was also compared between kale (Brassica oleracea var. acephala) and Ethiopian mustard using gas chromatography mass spectrometry (GC-MS). Laboratory choice tests showed that B. brassicae significantly preferred Ethiopian mustard (56.5%) to kale leaf discs (24.5%). Headspace GC-MS analysis revealed that kale emitted VOCs in higher concentrations. Among the 19 compounds detected in the two plants, only p-xylene, limonene, and α-pinene showed significant differences. Further, non-metric multidimensional scaling (NMDS) analysis revealed a significant difference between the overall VOCs emitted by the two plants (P < 0.05). Subsequently, field trials were conducted for two cropping seasons by separately intercropping kale with rosemary (Rosmarinus officinalis) and coriander (Coriandrum sativum) while bordering the two plots with Ethiopian mustard. In both seasons, the highest population of B. brassicae was observed on Ethiopian mustard bordering the C. sativum intercrop (P < 0.05). Our results indicate that Ethiopian mustard has the potential to be used as a trap crop in integrated pest management (IPM) strategies to protect kale against B. brassicae attack. DOI https://doi.org/10.1007/s11829-026-10246-5

Phylogeographic perspectives on conserving the endangered monotypic genus acanthochlamys (Velloziaceae)

Mon, 06 Apr 2026 00:00:00 GMT

Phylogeographic perspectives on conserving the endangered monotypic genus acanthochlamys (Velloziaceae) Wakhungu, Dennis A.; Li, Zhi-Peng; Kipkoech, Amos; Wambulwa, Moses C.; Oyebanji, Oyetola O.; Milne, Richard I.; Dudley, Christopher; Mambo, Winnie W.; Xu, Bo; Liu, Jie The accelerating loss of global biodiversity necessitates proactive conservation strategies that integrate genetic diversity with projected climate impacts, especially in biodiversity hotspots such as the Hengduan Mountains. While previous phylogeographic studies in this region primarily relied on a few DNA fragments, plastid phylogeography has seldom been integrated with ecological niche modelling for conservation planning. Here, we combined plastid phylogeography and ecological niche modelling using data from 107 individuals across 14 populations of Acanthochlamys bracteata, a vulnerable alpine species and monotypic genus, to identify genetically distinct populations and predict potential climatic refugia. We identified three genetically distinct groups (Groups A to C), all characterised by low nucleotide diversity but high haplotype endemism. Notably, Group C comprised a single population from Luhuo County, Sichuan Province, and represents an independent evolutionarily significant unit due to its pronounced genetic distinctiveness. Most genetic variation occurred among populations, with one population showing relatively high diversity, while three lacked variation. A northward range shift and expansion of suitable habitats are projected under future climatic scenarios. Three climatic refugia were identified, characterized by divergent genetic lineages and numerous private haplotypes, and supported by the persistence of suitable habitat through time. The observed phylogeographic structure is likely driven by both geographical distance and relict persistence. We recommend that conservation efforts should focus on protecting genetically distinct and diverse populations through an integration of in situ, ex situ, and assisted migration measures. This study advances our understanding of the evolutionary history of Acanthochlamys bracteata and could serve as a model for conserving other rare and isolated plants under climate change and human disturbance. https://doi.org/10.1007/s12686-026-01423-y

Investigation of rejuvenation and modification of aged asphalt binders by using aromatic oil-SBS polymer blend

Mon, 20 Jan 2020 00:00:00 GMT

Investigation of rejuvenation and modification of aged asphalt binders by using aromatic oil-SBS polymer blend Hong, Wei; Mo, Liantong; Pan, Changluan; Riara, Martin M.; Wei, Mi; Zhang, Jizhe In this paper, long-term aged base asphalt and SBS modified asphalt binders were rejuvenated and modified simultaneously using a compound rejuvenator containing 77% aromatic oil and 23% SBS polymer. Aromatic oil was used for rejuvenation purpose due to its softening effect while SBS polymer was used for modification purpose because of its potential to form a polymer network. Conventional bitumen tests, dynamic shear rheological (DSR) test and bending beam rheological (BBR) test were conducted to evaluate the rejuvenation and modification effects. Test results indicated that use of the compound rejuvenator enhanced the softening point and ductility for both aged base asphalt and SBS modified asphalt binders. The polymer modification effect of compound rejuvenator was well distinguished by the plateau region of phase angle master curves. Aromatic oil had a strong softening effect on aged asphalt binder which improved the performance at low temperatures, but weakened the performance at high temperatures. The incorporation of SBS polymer was a good supplement to balance the overall performance of rejuvenated asphalt binders. The compound rejuvenator can be greatly effective for hot recycling of reclaimed asphalt mixture by means of the simultaneous rejuvenation and modification. https://doi.org/10.1016/j.conbuildmat.2019.117154