dc.contributor.author |
Mukami, Asunta |
|
dc.contributor.author |
Ngetich, Alex |
|
dc.contributor.author |
Mweu, Cecilia |
|
dc.contributor.author |
Muthangya, Mutemi |
|
dc.contributor.author |
Oduor, Richard O. |
|
dc.contributor.author |
Ngugi, Mathew |
|
dc.contributor.author |
Mbinda, Wilton |
|
dc.date.accessioned |
2018-08-10T07:58:18Z |
|
dc.date.available |
2018-08-10T07:58:18Z |
|
dc.date.issued |
2018-07-18 |
|
dc.identifier.citation |
African journal of biotechnology, Vol. 17(29), pp. 898-905, 18 July, 2018 |
en_US |
dc.identifier.issn |
1684-5315 |
|
dc.identifier.uri |
http://www.academicjournals.org/journal/AJB/article-full-text-pdf/3EBE05657934 |
|
dc.identifier.uri |
http://repository.seku.ac.ke/handle/123456789/4260 |
|
dc.description |
DOI: 10.5897/AJB2018.16562 |
en_US |
dc.description.abstract |
A simple and efficient plant regeneration system via direct organogenesis was established in finger
millet using in vitro derived shoot apical meristems. Six varieties; GBK-043128, GBK-043094, GBK043050,
GBK-043137, GBK-043122 and GBK-043124 were evaluated. MS medium was used for
cotyledonary germination. Maximum number of shoots (84.33%) was observed in variety GBK-043128
while GBK-043094 had the least germination efficiency (62. 67%). Shoot apical meristems from threeday
old seedlings were evaluated for their potency of shoot induction on varied 6-benzylaminopurine
(BAP) concentrations. Highest shoot induction was observed in medium supplemented with 1.75 mg/l
BAP in GBK-043050 (3.00) whereas GBK-043094 (1.28) had the least response in medium supplemented
with 1.0 mg/l BAP. To induce rooting, in vitro regenerated plants cultured on MS medium was
supplemented with different concentrations of indole-3- acetic acid. The highest response in root
induction, with a larger number of roots (10.28), was observed in MS medium supplemented with 4.0 µM
IAA. Statistical analysis indicated that plant regeneration response varied greatly among the varieties.
In vitro germinated plants were successfully transferred to the greenhouse after hardening, with 300
shoots developing into fertile plants, which were indistinguishable with wild type plants. This plant
regeneration system has potential for production of transgenic finger millet crops. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Academic Journals |
en_US |
dc.subject |
Direct organogenesis |
en_US |
dc.subject |
finger millet |
en_US |
dc.subject |
root induction |
en_US |
dc.subject |
shoot apical meristems |
en_US |
dc.title |
Rapid and efficient plant regeneration from shoot apical meristems of finger millet [Eleusine coracana (L.) Gaertn.] via direct organogenesis |
en_US |
dc.type |
Article |
en_US |