dc.contributor.author |
Ng'ang'a, Zipporah W. |
|
dc.contributor.author |
Kimotho, James |
|
dc.contributor.author |
Nyairo, Edna |
|
dc.contributor.author |
Ochwoto, Missiani |
|
dc.contributor.author |
Nzioka, Nicholas |
|
dc.contributor.author |
Ogolla, Francis |
|
dc.contributor.author |
Kiptoo, Michael K. |
|
dc.date.accessioned |
2015-12-08T08:03:03Z |
|
dc.date.available |
2015-12-08T08:03:03Z |
|
dc.date.issued |
2015 |
|
dc.identifier.citation |
African Journal of Pharmacology and Therapeutics; Vol 4, No 3, 101-111 |
en_US |
dc.identifier.issn |
2303-9841 |
|
dc.identifier.uri |
http://journals.uonbi.ac.ke/ajpt/article/download/1384/1152 |
|
dc.identifier.uri |
http://repository.seku.ac.ke/handle/123456789/1846 |
|
dc.description.abstract |
Background: Human Immunodeficiency Virus (HIV) is characterized by high rates of genetic variability in vivo that could affect the performance of the HIV antibody-based detection kits.
Objective: This study aimed at developing immunoassays for HIV based on Consensus env gp41 Immunodominant region (IDR) from HIV infections in Kenya.
Methods: HIV RNA was extracted from 91 samples collected from 5 regional blood transfusion centers in Kenya. The RNA was reverse transcribed, sequenced in the env gp41-Immunodominant Region (IDR) and the Consensus sequence generated used to synthesize corresponding peptide. The Global HIV envgp41-IDR Consensus peptide was obtained from the literature and also synthesized. The two peptides were used to separately develop HIV immunoassays based on Enzyme-linked Immunosorbent Assay (ELISA) and Lateral Flow Assay (LFA) platforms and the performance of developed assays was evaluated. The same HIV env gp41 IDR peptides were used to develop ELISA-based immunoassays for determination HIV Incidence / Recency.
Results: The study did not find significant difference between the performance of the immunoassays that were developed with Consensus env gp41-IDR peptide (Kenya) and those developed using Consensus env gp41-IDR peptide (Global). However, the study found a significant difference between the performance of HIV ELISA for HIV Incidence testing that was developed with Consensus envgp41-IDR peptide (Kenya) and that which was developed using Consensus envgp41-IDR peptide (Global) with the former displaying superior performance.
Conclusions: The developed immunoassays demonstrated that both Consensus env gp41-IDR peptides (Kenya and Global) could be used to develop HIV immunoassays but Consensus env gp41-IDR peptide (Kenya) could be more suitable for development of HIV Incidence assays in Kenya. |
en_US |
dc.language.iso |
en |
en_US |
dc.subject |
HIV |
en_US |
dc.subject |
Consensus sequence |
en_US |
dc.subject |
env gp41-Immunodominant Region |
en_US |
dc.subject |
Immunoassays |
en_US |
dc.title |
Development of immunoassays for detection of Human Immunodeficiency Virus based on Consensus env gp41 Immunodominant Region Peptide from HIV-1 infections in Kenya |
en_US |
dc.type |
Article |
en_US |