dc.contributor.author |
Owino, Joseph H. |
|
dc.contributor.author |
Arotiba, Omotayo A. |
|
dc.contributor.author |
Hendricks, Nicolette |
|
dc.contributor.author |
Songa, Everlyne A. |
|
dc.contributor.author |
Jahed, Nazeem |
|
dc.contributor.author |
Waryo, Tesfaye T. |
|
dc.contributor.author |
Ngece, Rachel F. |
|
dc.contributor.author |
Baker, Priscilla G. |
|
dc.contributor.author |
Iwuoha, Emmanuel I. |
|
dc.date.accessioned |
2015-07-30T07:54:45Z |
|
dc.date.available |
2015-07-30T07:54:45Z |
|
dc.date.issued |
2008 |
|
dc.identifier.citation |
Sensors 2008, 8(12), 8262-8274 |
en_US |
dc.identifier.issn |
1424-8220 |
|
dc.identifier.uri |
http://www.mdpi.com/1424-8220/8/12/8262/pdf?view=inline |
|
dc.identifier.uri |
http://repository.seku.ac.ke/handle/123456789/1351 |
|
dc.description |
doi:10.3390/s8128262 |
en_US |
dc.description.abstract |
An aflatoxin B1 (AFB1) electrochemical immunosensor was developed by the immobilisation of aflatoxin B1-bovine serum albumin (AFB1-BSA) conjugate on a polythionine (PTH)/gold nanoparticles (AuNP)-modified glassy carbon electrode (GCE). The surface of the AFB1-BSA conjugate was covered with horseradish peroxidase (HRP), in order to prevent non-specific binding of the immunosensors with ions in the test solution. The AFB1 immunosensor exhibited a quasi-reversible electrochemistry as indicated by a cyclic voltammetric (CV) peak separation (ΔEp) value of 62 mV. The experimental procedure for the detection of AFB1 involved the setting up of a competition between free AFB1 and the immobilised AFB1-BSA conjugate for the binding sites of free anti-aflatoxin B1 (anti-AFB1) antibody. The immunosensor's differential pulse voltammetry (DPV) responses (peak currents) decreased as the concentration of free AFB1 increased within a dynamic linear range (DLR) of 0.6 - 2.4 ng/mL AFB1 and a limit of detection (LOD) of 0.07 ng/mL AFB1. This immunosensing procedure eliminates the need for enzyme-labeled secondary antibodies normally used in conventional ELISA–based immunosensors. |
en_US |
dc.language.iso |
en |
en_US |
dc.subject |
Immunosensor |
en_US |
dc.subject |
Gold nanoparticles |
en_US |
dc.subject |
Aflatoxin B1 |
en_US |
dc.subject |
Polythionine |
en_US |
dc.subject |
Horseradish peroxidise (HRP) |
en_US |
dc.title |
Electrochemical Immunosensor Based on Polythionine/Gold Nanoparticles for the Determination of Aflatoxin B1 |
en_US |
dc.type |
Article |
en_US |
dcterms.publisher |
MDPI |
|