dc.contributor.author |
Kinyua, Joyceline G. |
|
dc.contributor.author |
Lihana, Raphael W. |
|
dc.contributor.author |
Kiptoo, Michael K. |
|
dc.contributor.author |
Muasya, Timothy |
|
dc.contributor.author |
Odera, Irene |
|
dc.contributor.author |
Muiruri, Peter |
|
dc.contributor.author |
Songok, Elijah M. |
|
dc.date.accessioned |
2018-04-20T09:26:59Z |
|
dc.date.available |
2018-04-20T09:26:59Z |
|
dc.date.issued |
2018-04-02 |
|
dc.identifier.citation |
The Pan African Medical Journal. 2018;29:186. |
en_US |
dc.identifier.uri |
http://www.panafrican-med-journal.com/content/article/29/186/full/ |
|
dc.identifier.uri |
http://repository.seku.ac.ke/handle/123456789/4155 |
|
dc.description |
doi:10.11604/pamj.2018.29.186.10796 |
en_US |
dc.description.abstract |
Introduction: antiretroviral therapy plays a major role in reducing the impact of Human Immunodeficiency Virus/Acquired Immune Disease Syndrome, especially in resource-limited settings. However, without proper infrastructure, it has resulted in emergence of drug resistance mutations in infected populations. To determine drug resistance mutations among patients attending a comprehensive care facility in Nairobi, 65 blood samples were successfully sequenced.
Methods: whole blood samples were also tested for CD4+T-cell count and plasma HIV-1 RNA Viral load. Drug-resistance testing targeting the HIV-1 RT gene was determined. Patients were on first line ART that consisted of two NRTIs, and one NNRTI.
Results: females were younger (mean 42) than males (mean 45) and lower median CD4+ counts (139 cells/µl) than males (152 cells/µl). The prevalence of drug resistance mutations (any major mutation) in this population was 23.1% (15/65). Major NRTI mutations were detected in 11 patient samples, which included M184V (n = 6), M41L (n=3), D67N (n=2), K219Q (n=3) and T215F (n=2). Major NNRTI mutations were detected in 14 patient samples. They included K103N (n = 10), G190A (n = 1), Y181C (n = 1) and Y188L (n = 1).
Conclusion: presence of major mutations in this study calls for proper laboratory infrastructure to monitor treatment as well as regular appraisals of available regimens. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
African Field Epidemiology Network |
en_US |
dc.title |
Antiretroviral resistance among HIV-1 patients on first-line therapy attending a comprehensive care clinic in Kenyatta National Hospital, Kenya: a retrospective analysis |
en_US |
dc.type |
Article |
en_US |