dc.contributor.author |
Muturi, S. M. |
|
dc.contributor.author |
Karanja, L. S. |
|
dc.contributor.author |
Wachira, Francis N. |
|
dc.contributor.author |
Wambulwa, Moses C. |
|
dc.date.accessioned |
2017-08-23T09:00:50Z |
|
dc.date.available |
2017-08-23T09:00:50Z |
|
dc.date.issued |
2013-09 |
|
dc.identifier.citation |
African Journal of Biotechnology, Vol. 12(39), pp. 5790-5798, 25 September, 2013 |
en_US |
dc.identifier.issn |
1684-5315 |
|
dc.identifier.uri |
https://www.researchgate.net/publication/263850157_Ten_10_M_Ammonium_acetate_is_an_efficient_molecular_concentration_for_the_extraction_of_genomic_DNA_from_small_insects_used_for_rolling_circle_amplification |
|
dc.identifier.uri |
http://repository.seku.ac.ke/handle/123456789/3515 |
|
dc.description |
DOI: 10.5897/AJB2013.12848 |
en_US |
dc.description.abstract |
Most of the published methods for DNA isolation from vectors (insects) are not always effective in mealy-bugs, aphids and leafhoppers because they have high amount of polyphenols in their body systems. A simple, rapid, reliable labor-effective and carcinogenic compound-free salting out method for isolation of high molecular DNA from these insects is described. The ratio of OD260/OD280 ranged between 1.8 to 2.0. The method was successfully applied to Rolling Circle Amplification and in restriction analysis, indicating the removal of the common inhibitors. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Academic Journals |
en_US |
dc.subject |
Pseudococcidae |
en_US |
dc.subject |
Aphididae |
en_US |
dc.subject |
Auchenorryncha |
en_US |
dc.subject |
DNA extraction |
en_US |
dc.subject |
rolling circle amplification |
en_US |
dc.subject |
restriction analysis |
en_US |
dc.title |
Ten (10) M Ammonium acetate is an efficient molecular concentration for the extraction of genomic DNA from small insects used for rolling circle amplification |
en_US |
dc.type |
Article |
en_US |