Genetic diversity of African sorghum ( Sorghum bicolor L. Moench ) accessions based on microsatellite marker

Show simple item record Kimani, Paul M. Wachira, Francis N. Cheruiyot, Erick K. Owuoche, James Esther, Kimani 2017-08-22T08:18:01Z 2017-08-22T08:18:01Z 2014
dc.identifier.citation Australian Journal of Crop Science,Vol.8 No.2 pp.171-177 ref.25 2014 en_US
dc.identifier.issn 1835-2693
dc.description.abstract Genetic diversity plays an important role in selection of parental stocks in plant breeding. Sorghum [Sorghum bicolor (L.) Moench] breeding initiatives have been constrained by lack of information on genetic diversity of cultivated accessions which would otherwise guide in the choice of heterotic parents for hybridization. This study was carried out to determine genetic diversity of sorghum accessions collected from Africa and ICRISAT using simple sequence repeats (SSR) of microsatellite marker. Thirty sorghum SSR markers were used to assess the genetic diversity of 134 sorghum accessions. The number of alleles per microsatellite locus in the 134 sorghum accessions ranged between 2 to 22, with a total of 259 different alleles having been amplified. The greatest number of alleles was found at the locus 3590e705f67911e0b58c0010185a4b14 with 22 alleles. The average Polymorphism Information Content (PIC) for all the assayed sorghum accessions was 0.55. Expected heterozygosity of population ranged between 2.91 for Sudan and 1.58 for Central Africa. Pairwise population comparisons for genetic identity were derived based on Nei's. Genetic identity of the populations ranged from 0.36 for Central Africa and Northern Africa to 0.93 between Eastern Africa and Rwanda. Analysis of Molecular Variance (AMOVA) revealed that 75% of the molecular variation in sorghum accessions was due to within individual populations while 25% of the total variation was partitioned among populations. There was low population differentiation due to either continuous exchange of genes between sorghum populations largely by germplasm exchange or no intense selection processes. The diversity observed within sorghum accession from Sudan and East African region could be useful in improvement of sorghum for various traits. en_US
dc.language.iso en en_US
dc.subject genetic diversity en_US
dc.subject identity en_US
dc.subject sorghum en_US
dc.title Genetic diversity of African sorghum ( Sorghum bicolor L. Moench ) accessions based on microsatellite marker en_US
dc.type Article en_US

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