Investigation of the presence and antinociceptive function of muscarinic acetylcholine receptors in the African naked mole-rat (Heterocephalus glaber)

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dc.contributor.author Kanui, Titus I.
dc.contributor.author Jørgensen, K. B.
dc.contributor.author Krogh-Jensen, K.
dc.contributor.author Pickering, D. S.
dc.contributor.author Abelson, K. S.
dc.date.accessioned 2015-11-11T06:01:52Z
dc.date.available 2015-11-11T06:01:52Z
dc.date.issued 2016-01
dc.identifier.citation J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2015 Oct 31; pp 1-9 en_US
dc.identifier.issn 1432-1351
dc.identifier.issn 0340-7594
dc.identifier.uri http://download.springer.com/static/pdf/113/art%253A10.1007%252Fs00359-015-1048-x.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00359-015-1048-x&token2=exp=1447222732~acl=%2Fstatic%2Fpdf%2F113%2Fart%25253A10.1007%25252Fs00359-015-1048-x.pdf%3ForiginUrl%3Dhttp%253A%252F%252Flink.springer.com%252Farticle%252F10.1007%252Fs00359-015-1048-x*~hmac=32a2a184910c942bb72b8110e5a10743f10475803ff01877a33eeb5b7702bc9a
dc.identifier.uri http://repository.seku.ac.ke/handle/123456789/1829
dc.description DOI: 10.1007/s00359-015-1048-x en_US
dc.description.abstract The present study investigated the cholinergic system in the African naked mole-rat (Heterocephalus glaber) with focus on the muscarinic acetylcholine receptor subtypes M1 and M4. The protein sequences for the subtypes m 1-5 of the naked mole-rat were compared to that of the house mouse (Mus musculus) using basic local alignment search tool (BLAST). The presence and function of M1 and M4 was investigated in vivo, using the formalin test with the muscarinic receptor agonists xanomeline and VU0152100. Spinal cord tissue from the naked mole-rat was used for receptor saturation binding studies with [3H]-N-methylscopolamine. The BLAST test revealed 95 % protein sequence homology showing the naked mole-rat to have the genetic potential to express all five muscarinic acetylcholine receptor subtypes. A significant reduction in pain behavior was demonstrated after administration of 8.4 mg/kg in the formalin test. Administration of 50 mg/kg VU0152100 resulted in a non-significant tendency towards antinociception. The antinociceptive effects were reversed by the muscarinic acetylcholine receptor antagonist atropine. Binding studies indicated presence of muscarinic acetylcholine receptors with a radioligand affinity comparable to that reported in mice. In conclusion, muscarinic acetylcholine receptor subtypes are present in the naked mole-rat and contribute to antinociception in the naked mole-rat. en_US
dc.language.iso en en_US
dc.publisher Springer Berlin Heidelberg en_US
dc.subject Cholinergic receptors en_US
dc.subject Muscarinic en_US
dc.subject antinociception en_US
dc.subject formalin test en_US
dc.subject Naked mole-rat en_US
dc.title Investigation of the presence and antinociceptive function of muscarinic acetylcholine receptors in the African naked mole-rat (Heterocephalus glaber) en_US
dc.type Article en_US


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